| 七夕结缘！蛋白质红娘Matchmaker® 前来助力! | |
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纤云弄巧，飞星传恨，银汉迢迢暗渡。 金风玉露一相逢，便胜却人间无数。 柔情似水，佳期如梦，忍顾鹊桥归路。 两情若是久长时，又岂在朝朝暮暮。 |
| 《鹊桥仙·纤云弄巧》秦观 | |
| 又是一年七夕节，相传在每年的七夕，成千上万只喜鹊会从四面八方簇拥在银河，搭起一座鹊桥，让牛郎织女团聚。七夕节又被称为中国的“情人节”，在这样一个节日里，年轻人都祈祷拥有美好的姻缘。但还有些孤独的蛋白质小精灵，要怎么才能找到和自己互作的另一半并和他们团聚呢？ | |
| 幸好蛋白质小精灵们遇见了Matchmaker® Gold酵母双杂交系统！Takara旗下Clontech品牌的酵母杂交产品线作为生命科学研究界的“Matchmaker （红娘）”，致力于为天下的互作蛋白质牵引姻缘的“红线”，搭起相见的“鹊桥”。Matchmaker® Gold已经帮助了很多蛋白质小精灵在茫茫人海中找到了与他们互作的另一半！ | |
| 下面就让我们来看一看Matchmaker® Gold酵母双杂交系统为搭起蛋白质小精灵之间的“鹊桥”做了哪些努力吧。 | |
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| 第一步 提供文库，不轻易错过可能互作的蛋白质！ | |
| 均一化的酵母双杂交cDNA文库—Normalized Mate & Plate Libraries，筛选不再大海捞针！ | |
| 特点 | |
| 1. 为蛋白质互作中的文库筛选提供简便方法。 2. 筛选更少的克隆，检测更多的互作。 3. 通用型文库——更高的基因代表性。 |
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| 没有选到合适的文库?没关系！我们还有Make Your Own "Mate & Plate™" Library（630490），制作您自己的文库，简便而且快速！ | |
| 酵母双杂交文库构建系统，您可以根据需求自己构建酵母文库，配合Matchmaker® Gold酵母双杂交系统可以从百万级别的文库中找出具有相互作用的蛋白质！ | |
| 特点 | |
| 1. 样本量要求低--Total RNA起始即可，最低起始量仅需100 ng，真核生物样本均可构建。 2. 建库简便-SMART技术与同源重组技术相结合构建文库，聪明的酵母自己做克隆。 3. 性价比高-可用于数百次的双杂交筛选实验。 |
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| 成功案例 | |
| 植物研究： | |
| 1. Dou, L., He, K., et al. (2021). The E3 ligase MREL57 modulates microtubule stability and stomatal closure in response to ABA. Nature communications, 12(1), 2181. IF：14.91 2. Lu, R., Dong, Y., et al. (2020). Necdin regulates BMAL1 stability and circadian clock through SGT1-HSP90 chaperone machinery. Nucleic acids research, 48(14), 7944-7957. IF: 16.971 3. Furutani, M., Hirano, Y., et al. (2020). Polar recruitment of RLD by LAZY1-like protein during gravity signaling in root branch angle control. Nature communications, 11(1), 76. IF: 14.919 4. Schwarzenbacher, R. E., Wardell, G., et al. (2020). The IBI1 Receptor of β-Aminobutyric Acid Interacts with VOZ Transcription Factors to Regulate Abscisic Acid Signaling and Callose-Associated Defense. Molecular plant, 13(10), 1455-1469. IF: 13.16 |
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| 动物研究： | |
| 1. Felipe-Medina, N., Caburet, S., et al. (2020). A missense in HSF2BP causing primary ovarian insufficiency affects meiotic recombination by its novel interactor C19ORF57/BRME1. eLife, 9, e56996. IF：8.14 2. Stanic, J., Carta, M., et al. (2015). Rabphilin 3A retains NMDA receptors at synaptic sites through interaction with GluN2A/PSD-95 complex. Nature communications, 6, 10181. IF: 14.91 |
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| 医学研究： | |
| 1. Feng, Q., Bian, X., et al. (2020). Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy. BMC cancer, 20(1), 665. IF：4.43 2. Rafizadeh, S., Zhang, Z., et al. (2014). Functional interaction with filamin A and intracellular Ca2+ enhance the surface membrane expression of a small-conductance Ca2+-activated K+ (SK2) channel. Proceedings of the National Academy of Sciences of the United States of America, 111(27), 9989-9994. IF：11.205 |
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| 第二步 文库筛选，在茫茫人海中找到“真爱”！ | |
| Matchmaker® Gold Yeast Two-Hybrid System（630489），可以从百万级别的文库中筛选到互作蛋白质。 | |
| 特点 | |
| 1. 操作简单-筛选酵母阳性克隆，轻松找到目的蛋白质的另一半！ 2. 降低“假阳性”-3个启动子，4个报告基因（包括 Aureobasidin A（AbA）抗生素抗性筛选基因），为您强有力的筛选掉“假阳性”！ 3. 筛选效率高-简便的“Mating”，酵母接合方法，提高筛选效率！ |
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| 成功案例 | |
| 植物研究： | |
| 1. Wang, P., Jiang, H., et al. (2021). A nuclear-targeted effector of Rhizophagus irregularis interferes with histone 2B mono-ubiquitination to promote arbuscular mycorrhisation. The New phytologist, 230(3), 1142-1155. IF: 10.151 2. Xu, Y., Zhang, L., et al. (2020). Natural variations of SLG1 confer high-temperature tolerance in indica rice. Nature communications, 11(1), 5441. IF: 14.919 3. Xu, G., Zhong, X., et al. (2020). A fungal effector targets a heat shock-dynamin protein complex to modulate mitochondrial dynamics and reduce plant immunity. Science advances, 6(48), eabb7719. IF: 14.136 4. Reddy, M. R., Mangrauthia, S. K., et al. (2020). PAP90, a novel rice protein plays a critical role in regulation of D1 protein stability of PSII. Journal of advanced research, 30, 197-211. IF: 10.479 |
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| 医学研究： | |
| 1. Shi, W., Zhang, G., et al. (2021). Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer. Nature communications, 12(1), 2812. IF: 14.919 2. Pathak, P., Blech-Hermoni., et al. (2021). Myopathy associated LDB3 mutation causes Z-disc disassembly and protein aggregation through PKCα and TSC2-mTOR downregulation. Communications biology, 4(1), 355. IF：6.268 3. Khongwichit, S., Sornjai, W., et al. (2021). A functional interaction between GRP78 and Zika virus E protein. Scientific reports, 11(1), 393. IF: 4.379 4. Wang, X., Jiang, W., et al. (2021). The C. elegans homolog of human panic-disorder risk gene TMEM132D orchestrates neuronal morphogenesis through the WAVE-regulatory complex. Molecular brain, 14(1), 54. IF:4.041 |
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| 微生物研究： | |
| 1. Ludwig, N., Reissmann, S., et al. (2021). A cell surface-exposed protein complex with an essential virulence function in Ustilago maydis. Nature microbiology, 6(6), 722-730. IF: 17.745 2. Yang, C., Hu, B., et al. (2020). State changes of the HORMA protein ASY1 are mediated by an interplay between its closure motif and PCH2. Nucleic acids research, 48(20), 11521-11535. IF: 16.971 |
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| 最后一步 互作验证，确定是彼此的“真爱”！ | |
| Matchmaker Mammalian Assay Kit 2（630305）， 验证哺乳动物来源的互作蛋白质。 | |
| 特点 | |
| 1. 在哺乳动物细胞内简便迅速地分析蛋白质之间的互作。 2. 分泌型的碱性磷酸酶SEAP报告子避免了细胞裂解的繁琐操作。 3. 分析结果更加真实。 |
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| 成功案例： | |
| 1. Ohkuni, K., Pasupala, N., et al. (2018). SUMO-Targeted Ubiquitin Ligases (STUbLs) Reduce the Toxicity and Abnormal Transcriptional Activity Associated With a Mutant, Aggregation-Prone Fragment of Huntingtin. Frontiers in genetics, 9, 379. IF：4.599 2. Huang, Y., Ma, D., et al. (2017). Interaction between HCMV pUL83 and human AIM2 disrupts the activation of the AIM2 inflammasome. Virology journal, 14(1), 34. IF：4.099 3. Laurenzana, E. M., Coslo, D. M., et al. (2016). Activation of the Constitutive Androstane Receptor by Monophthalates. Chemical research in toxicology, 29(10), 1651-1661.IF：3.739 |
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| 想了解和学习更多Takara酵母杂交实验相关内容，请前往酵母杂交学习中心！ | |
页面更新：2021-08-12 15:35:43
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