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{"sheet":"Takara体外转录服务委托书","index":0,"n_rows":51,"n_cols":33,"rows_kept":51,"merged":[[1,1,1,41],[2,1,2,41],[4,2,4,14],[4,30,4,41],[6,1,6,6],[9,3,9,5],[9,8,9,11],[9,13,9,15],[9,17,9,19],[11,3,11,6],[12,3,12,5],[12,6,12,7],[12,8,12,19],[12,22,12,24],[12,26,12,36],[13,3,13,5],[13,6,13,7],[13,8,13,19],[13,22,13,24],[13,26,13,36],[14,3,14,5],[14,6,14,7],[14,8,14,19],[14,22,14,24],[14,26,14,36],[15,3,15,6],[15,17,15,22],[15,25,15,30],[16,4,16,12],[16,21,16,26],[16,27,16,36],[17,4,17,8],[17,9,17,18],[17,20,17,21],[17,22,17,25],[17,28,17,29],[17,30,17,36],[19,20,19,21],[19,22,19,25],[19,28,19,29],[19,30,19,36],[21,3,21,11],[24,3,25,3],[24,4,25,9],[24,10,25,14],[24,15,25,20],[24,21,25,26],[24,27,25,30],[24,31,25,36],[29,3,29,40],[32,3,32,5],[36,2,36,39],[36,54,37,90],[37,3,38,40],[39,3,40,40],[41,3,42,40],[43,3,44,40],[50,2,50,9],[51,1,51,2],[52,1,52,2],[53,1,53,2]],"flags":["formulas_cached_only"]}
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[null,"Takara 体外转录服务委托书"]
[null,"(Takara Custom Services)"]
[]
[null,null,"FAX:0411-87619946",{"$nulls":16},"Tel: 0411-87509077",{"$nulls":10},"E-mail: service10@takara.com.cn"]
[]
[null,"尊敬的客户："]
[{"$nulls":3},"您好！为了顺利地完成实验，请您认真阅读“体外转录”技术服务说明后，填写以下内容："]
[]
[null,null,"1.","委托日",{"$nulls":8},"年",{"$nulls":3},"月",{"$nulls":3},"日"]
[]
[null,null,"2.","委托人情况"]
[{"$nulls":3},"姓名",null,null,"：",{"$nulls":15},"单位名称",null,null,"："]
[{"$nulls":3},"固定电话",null,null,"：",{"$nulls":15},"移动电话",null,null,"："]
[{"$nulls":3},"E-mail",null,null,"：",{"$nulls":15},"代 理 商 ",null,null,"："]
[null,null,"3.","服务项目"]
[{"$nulls":4},"转录模板的制备"]
[{"$nulls":4},"RNA合成"]
[{"$nulls":4},"合成后样本的调制"]
[{"$nulls":6},"RNA标准品的制备，具体要求："]
[{"$nulls":6},"其他要求："]
[null,null,"4.","背景资料"]
[{"$nulls":3},"模板形式：",{"$nulls":5},"环形质粒",{"$nulls":6},"人工合成基因",{"$nulls":6},"  PCR产物"]
[]
[{"$nulls":3},"No.","样本名称",{"$nulls":5},"浓度\n(ng/μl)",{"$nulls":4},"体积(μl)",{"$nulls":5},"总量(μg)",{"$nulls":5},"酶切位点*",{"$nulls":3},"备注"]
[]
[{"$nulls":3},1]
[{"$nulls":3},2]
[{"$nulls":3},3]
[{"$nulls":3},"*指提供环形质粒时，线性化质粒需使用的酶切位点。"]
[{"$nulls":3},"请简单说明合成RNA的用途：",{"$nulls":11},"qPCR标准品",{"$nulls":6},"Pulldown",{"$nulls":3},"基因组编辑",{"$nulls":5},"其他用途"]
[]
[null,null,"5.","提供结果"]
[{"$nulls":4},"电子版实验报告（包含实验方法、电泳照片等）和转录后RNA制品；"]
[{"$nulls":4},"其他特殊要求请说明:                                                        "]
[]
[null,null,"【注意事项】"]
[null,null,"1.","目前接收的样品形式主要为：A、含有T7 promotor或SP6 promotor的质粒或PCR产物。要求样品浓度≥200 ng/μl，总量≥10 μg。B、提供序列，我们为您人工合成含promotor的质粒。"]
[]
[null,null,"2.","以环形质粒为模板进行体外转录，合成的RNA为长短不同的混合物；以质粒酶切产物或PCR产物为模板进行体外转录，合成的RNA为确定长度。请根据您的实验目的，正确选择模板形式。"]
[]
[null,null,"3.","体外转录后的RNA中会混有部分模板DNA，使用DNase I处理后会减少模板DNA的污染。若，RNA制品，您后续用于qPCR的检测，我们也可以提供残留DNA的质检。"]
[]
[null,null,"4.","对于非本公司测序的质粒样品，我们首先要进行测序验证，并收取相应的测序费用。请您将目的序列的电子版信息及测序引物信息发送到我们的邮箱service10@takara.com.cn中。"]
[]
[null,null,"5. 我们不接受人源样品（含血液、组织、细胞、切片、核酸等）及有致病性的样品。"]
[]
[{"$nulls":3},"请您确认所寄样品非人源且无致病性。",{"$nulls":19},"确认签名栏："]
[]
[]
[null,null,"附加信息（参考序列）"]