Takara

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T7 RNA Polymerase, HQ的使用例

Sterile purified water	X μl
10X T7 RNA Polymerase Buffer	2 μl
ATP, CTP, GTP, UTP	each 10 mM
Template DNA	0.5 - 2 μg
Recombinant RNase Inhibitor, HQ	20 U
Pyrophosphatase (inorganic), HQ	0.1 U
T7 RNA Polymerase, HQ	200 U
Total	20 μl

37℃ 下孵育 1-2 小时。