| >> 升级的无缝克隆试剂In-Fusion Snap Assembly | ||||
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| >> 看动画，让你更懂In-Fusion无缝克隆 | ||||
| 2分钟了解In-Fusion的操作流程 | ||||
| 多才多艺的In-Fusion无缝克隆 | ||||
| 2分钟看懂In-Fusion作用机制的与众不同 | ||||
| >> In-Fusion客户应用实例 | ||||
| ■ 利用In-Fusion Cloning插入小的融合蛋白质标签 | ||||
| ■ In-Fusion Cloning：连接酶的高效率高准确度替代方法 | ||||
| ■ In-Fusion Cloning：高效率的单片段和多片段克隆 | ||||
| ■ In-Fusion Cloning：成功地直接克隆到大表达载体 | ||||
| ■ In-Fusion Cloning：克服慢病毒载体酶切位点的限制 | ||||
| ■ In-Fusion Cloning：更快速地得到比TOPO克隆更准确的结果 | ||||
| ■ In-Fusion Cloning：简单的多片段克隆解决一个合成的难题 | ||||
| ■ 利用In-Fusion Cloning System克隆2个PCR片段至载体 | ||||
| ■ 利用In-Fusion Cloning System进行3个插入片段与载体的多片段克隆 | ||||
| ■ 利用In-Fusion 方法进行4个插入片段与载体的多片段克隆 | ||||
| ■ In-Fusion Cloning Kit与其他公司同类型试剂盒的比较 | ||||
| ■ 使用In-Fusion Cloning System构建基因编辑敲除用供体载体（3个插入片段的In-Fusion克隆） | ||||
| >> In-Fusion应用文献例 | ||||
| ■ CRISPR/Cas9载体克隆 | ||||
| · Efficient genomic correction methods in human iPS cells using CRISPR-Cas9 system | ||||
| · A highly efficient ligation-independent cloning system for CRISPR/Cas9 based genome editing in plants |
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| · MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems |
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| ■ 抗体工程研究中的高通量克隆 | ||||
| · Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells | ||||
| ■ 慢病毒载体克隆 | ||||
| · Gene expression profiling reveals U1 snRNA regulates cancer gene expression | ||||
| · Single cell resolution in vivo imaging of DNA damage following PARP inhibition | ||||
| · Lrig1 is a haploinsufficient tumor suppressor gene in malignant glioma. Self-targeting of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors |
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| · Self-targeting of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors |
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| ■ 合成DNA组装 | ||||
| · Initiation of translation in bacteria by a structured eukaryotic IRES RNA | ||||
| · Targeted mutagenesis of guinea pig Cytomegalovirus using CRISPR/Cas9-mediated gene editing |
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| ■ 高通量克隆 | ||||
| · A versatile ligation-independent cloning method suitable for high-throughput expression screening applications |
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| · Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells | ||||
| · High throughput generation and characterization of replication-competent clade C transmitter-founder simian human immunodeficiency viruses |
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| ■ 多片段克隆 | ||||
| · In-Fusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations |
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| · Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a variety of lipid-linked glycans |
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| · Multi-homologous recombination-based gene manipulation in the rice pathogen Fusarium fujikuroi |
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| · Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor |
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| · Structure-function studies of an engineered scaffold protein derived from Stefin A.II: Development and applications of the SQT variant |
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| · Regulating prospero mRNA stability determines when neural stem cells stop dividing | ||||
| · Identification of the N-terminal domain of the influenza virus PA responsible for the suppression of host protein synthesis |
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| · Root developmental programs shape the Medicago truncatula nodule meristem | ||||
| · Towards a systems approach in the genetic analysis of archaea: accelerating mutant construction and phenotypic analysis in Haloferax volcanii |
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| · Potential pitfalls and solutions for use of fluorescent fusion proteins to study the lysosome |
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| >> In-Fusion克隆工具箱 | ||||
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| In-Fusion引物设计工具 | In-Fusion体系摩尔比计算器 | |||
| >> 实验设计原则和技巧 | ||||
| ■ 利用In-Fusion HD Cloning进行碱基删除、插入或替换 | ||||
| ■ 利用In-Fusion HD Cloning进行多片段克隆的实验技巧 | ||||
| ■ In-Fusion HD Cloning技巧、诀窍、常见问题和排查 | ||||
| · FAQs | ||||
| · Tips | ||||
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