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当前位置:首页> 产品选择指南 > cDNA合成 & 克隆 > 克隆学习中心 > 高效的In-Fusion无缝克隆试剂盒
 
>> 升级的无缝克隆试剂In-Fusion Snap Assembly
 
 
 
 
 
 
>> 看动画,让你更懂In-Fusion无缝克隆
2分钟了解In-Fusion的操作流程
 
多才多艺的In-Fusion无缝克隆
 
2分钟看懂In-Fusion作用机制的与众不同
 
>> In-Fusion客户应用实例
    ■ 利用In-Fusion Cloning插入小的融合蛋白质标签
 
    ■ In-Fusion Cloning:连接酶的高效率高准确度替代方法
 
    ■ In-Fusion Cloning:高效率的单片段和多片段克隆
 
    ■ In-Fusion Cloning:成功地直接克隆到大表达载体
 
    ■ In-Fusion Cloning:克服慢病毒载体酶切位点的限制
 
    ■ In-Fusion Cloning:更快速地得到比TOPO克隆更准确的结果
 
    ■ In-Fusion Cloning:简单的多片段克隆解决一个合成的难题
 
    ■ 利用In-Fusion Cloning System克隆2个PCR片段至载体
 
    ■ 利用In-Fusion Cloning System进行3个插入片段与载体的多片段克隆
 
    ■ 利用In-Fusion 方法进行4个插入片段与载体的多片段克隆
 
    ■ In-Fusion Cloning Kit与其他公司同类型试剂盒的比较
 
    ■ 使用In-Fusion Cloning System构建基因编辑敲除用供体载体(3个插入片段的In-Fusion克隆)
 
>> In-Fusion应用文献例
    ■ CRISPR/Cas9载体克隆
    · Efficient genomic correction methods in human iPS cells using CRISPR-Cas9 system
    · A highly efficient ligation-independent cloning system for CRISPR/Cas9 based
       genome editing in plants
    · MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the
       PITCh systems
 
    ■ 抗体工程研究中的高通量克隆
    · Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells
 
    ■ 慢病毒载体克隆
    · Gene expression profiling reveals U1 snRNA regulates cancer gene expression
    · Single cell resolution in vivo imaging of DNA damage following PARP inhibition
    · Lrig1 is a haploinsufficient tumor suppressor gene in malignant glioma. Self-targeting
       of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors
    · Self-targeting of TNF-releasing cancer cells in preclinical models of primary and
       metastatic tumors
 
    ■ 合成DNA组装
    · Initiation of translation in bacteria by a structured eukaryotic IRES RNA
    · Targeted mutagenesis of guinea pig Cytomegalovirus using CRISPR/Cas9-mediated
       gene editing
 
    ■ 高通量克隆
    · A versatile ligation-independent cloning method suitable for high-throughput
       expression screening applications
    · Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells
    · High throughput generation and characterization of replication-competent clade C
       transmitter-founder simian human immunodeficiency viruses
 
    ■ 多片段克隆
    · In-Fusion assembly: seamless engineering of multidomain fusion proteins, modular
       vectors, and mutations
    · Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a
       variety of lipid-linked glycans
    · Multi-homologous recombination-based gene manipulation in the rice pathogen
       Fusarium fujikuroi
    · Structural basis of pathogen recognition by an integrated HMA domain in a plant
       NLR immune receptor
    · Structure-function studies of an engineered scaffold protein derived from Stefin
       A.II: Development and applications of the SQT variant
    · Regulating prospero mRNA stability determines when neural stem cells stop dividing
    · Identification of the N-terminal domain of the influenza virus PA responsible for
       the suppression of host protein synthesis
    · Root developmental programs shape the Medicago truncatula nodule meristem
    · Towards a systems approach in the genetic analysis of archaea: accelerating
       mutant construction and phenotypic analysis in Haloferax volcanii
    · Potential pitfalls and solutions for use of fluorescent fusion proteins to study
       the lysosome
 
>> In-Fusion克隆工具箱
In-Fusion引物设计工具 In-Fusion体系摩尔比计算器
 
>> 实验设计原则和技巧
    ■ 利用In-Fusion HD Cloning进行碱基删除、插入或替换
    ■ 利用In-Fusion HD Cloning进行多片段克隆的实验技巧
    ■ In-Fusion HD Cloning技巧、诀窍、常见问题和排查
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