>> 升级的无缝克隆试剂In-Fusion Snap Assembly | ||||
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>> 看动画,让你更懂In-Fusion无缝克隆 | ||||
2分钟了解In-Fusion的操作流程 | ||||
多才多艺的In-Fusion无缝克隆 | ||||
2分钟看懂In-Fusion作用机制的与众不同 | ||||
>> In-Fusion客户应用实例 | ||||
■ 利用In-Fusion Cloning插入小的融合蛋白质标签 | ||||
■ In-Fusion Cloning:连接酶的高效率高准确度替代方法 | ||||
■ In-Fusion Cloning:高效率的单片段和多片段克隆 | ||||
■ In-Fusion Cloning:成功地直接克隆到大表达载体 | ||||
■ In-Fusion Cloning:克服慢病毒载体酶切位点的限制 | ||||
■ In-Fusion Cloning:更快速地得到比TOPO克隆更准确的结果 | ||||
■ In-Fusion Cloning:简单的多片段克隆解决一个合成的难题 | ||||
■ 利用In-Fusion Cloning System克隆2个PCR片段至载体 | ||||
■ 利用In-Fusion Cloning System进行3个插入片段与载体的多片段克隆 | ||||
■ 利用In-Fusion 方法进行4个插入片段与载体的多片段克隆 | ||||
■ In-Fusion Cloning Kit与其他公司同类型试剂盒的比较 | ||||
■ 使用In-Fusion Cloning System构建基因编辑敲除用供体载体(3个插入片段的In-Fusion克隆) | ||||
>> In-Fusion应用文献例 | ||||
■ CRISPR/Cas9载体克隆 | ||||
· Efficient genomic correction methods in human iPS cells using CRISPR-Cas9 system | ||||
· A highly efficient ligation-independent cloning system for CRISPR/Cas9 based genome editing in plants |
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· MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems |
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■ 抗体工程研究中的高通量克隆 | ||||
· Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells | ||||
■ 慢病毒载体克隆 | ||||
· Gene expression profiling reveals U1 snRNA regulates cancer gene expression | ||||
· Single cell resolution in vivo imaging of DNA damage following PARP inhibition | ||||
· Lrig1 is a haploinsufficient tumor suppressor gene in malignant glioma. Self-targeting of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors |
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· Self-targeting of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors |
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■ 合成DNA组装 | ||||
· Initiation of translation in bacteria by a structured eukaryotic IRES RNA | ||||
· Targeted mutagenesis of guinea pig Cytomegalovirus using CRISPR/Cas9-mediated gene editing |
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■ 高通量克隆 | ||||
· A versatile ligation-independent cloning method suitable for high-throughput expression screening applications |
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· Rapid high-throughput cloning and stable expression of antibodies in HEK293 cells | ||||
· High throughput generation and characterization of replication-competent clade C transmitter-founder simian human immunodeficiency viruses |
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■ 多片段克隆 | ||||
· In-Fusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations |
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· Substrate promiscuity: AglB, the archaeal oligosaccharyltransferase, can process a variety of lipid-linked glycans |
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· Multi-homologous recombination-based gene manipulation in the rice pathogen Fusarium fujikuroi |
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· Structural basis of pathogen recognition by an integrated HMA domain in a plant NLR immune receptor |
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· Structure-function studies of an engineered scaffold protein derived from Stefin A.II: Development and applications of the SQT variant |
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· Regulating prospero mRNA stability determines when neural stem cells stop dividing | ||||
· Identification of the N-terminal domain of the influenza virus PA responsible for the suppression of host protein synthesis |
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· Root developmental programs shape the Medicago truncatula nodule meristem | ||||
· Towards a systems approach in the genetic analysis of archaea: accelerating mutant construction and phenotypic analysis in Haloferax volcanii |
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· Potential pitfalls and solutions for use of fluorescent fusion proteins to study the lysosome |
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>> In-Fusion克隆工具箱 | ||||
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In-Fusion引物设计工具 | In-Fusion体系摩尔比计算器 | |||
>> 实验设计原则和技巧 | ||||
■ 利用In-Fusion HD Cloning进行碱基删除、插入或替换 | ||||
■ 利用In-Fusion HD Cloning进行多片段克隆的实验技巧 | ||||
■ In-Fusion HD Cloning技巧、诀窍、常见问题和排查 | ||||
· FAQs | ||||
· Tips | ||||
页面更新:2021-11-17 15:49:05