应用 |
基因分型 |
长链PCR、测序、克隆 |
NGS前扩增 |
推荐产品 |
高GC PCR-
Advantage GC 2 |
TaKaRa LA Taq
with GC Buffer |
PrimeSTAR GXL DNA
Polymerase |
适用于 |
高GC |
GC-rich模板的长链扩增 |
高保真扩增 |
扩增片段大小 |
gDNA |
﹤3.5 kb |
﹤17.5 kb |
﹤30 kb |
Plasmid/lambda |
﹤6 kb |
﹤35 kb |
﹤40 kb |
cDNA |
﹤2 kb |
NA |
﹤13.5 kb |
制品性能 |
5'→3' exonuclease
activity |
|
√ |
|
3'→5' exonuclease
activity |
√ |
√ |
√ |
T/A overhangs or
blunt |
T/A |
T/A |
Blunt |
Fidelity (Kunkel) |
3X Taq (standard
%GC target) |
6.5X Taq
(standard %GC target) |
|
Fidelity (sequencing) |
|
|
6.5X Taq
(GC-rich target) |
Speed |
60 sec/kb |
60 sec/kb |
10 sec/kb
(rapid protocol) |
GC-rich targets |
Up to 90% |
Up to 74% |
73% |
包装形式/特征 |
PCR kit with control
template and primers |
可选 |
可选 |
|
Hot start |
|
|
√ |