| The Pluripotency Check PCR Kit用来通过PCR方法监控在发育多能性方面有关键作用的基因，从而判断小鼠胚胎干细胞或是iPS是否真实具有干细胞多能性（pluripotency）。本产品覆盖16个基因，包括9个在真实干细胞中表达的基因和2个内参基因。客户可以灵活决定使用哪些基因进行实际检测。本产品可以鉴别关键基因的表达是内源性的（endogenous expression）还是外源性的(exogenous expression)。 |
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| ■ Pluripotency Check PCR Primers |
| Primer set |
Amplicon size |
Primer set |
Amplicon size |
| Oct3/4 (Total) |
485 bp |
Klf4 |
739 bp |
| Oct3/4 (Endogenous) |
224 bp |
Klf4 (Endogenous) |
206 bp |
| Nanog (Total) |
364 bp |
Ecat1 |
164 bp |
| Nanog (Endogenous) |
223 bp |
ERas1 |
210 bp |
| Sox2 (Total) |
193 bp |
Esg1 |
376 bp |
| Sox2 (Endogenous) |
297 bp |
Rex1 |
287 bp |
| c-Myc (Total) |
228 bp |
Beta-Actin (Control) |
603 bp |
| c-Myc (Endogenous) |
170 bp |
GAPDH (Control) |
568 bp |
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| ■ 产品特点 |
Validate the pluripotent status of your stem cell or iPS cell culture using a simple PCR kit
· 16 prevalidated PCR primer mixes
· Complete kit with RNA extraction, reverse transcription, and PCR reagents
· Confirm your iPS cells by differentiating between endogenous and exogenous gene expression
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| ■ 产品应用 |
· Validating mouse embryonic stem cell status
· Confirming iPS status
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| Code No. |
Product |
Size |
| 631965 |
Pluripotency Check PCR Kit |
Each |
| 631966 |
Pluripotency Check PCR Primer Set |
16 × 25 Rxns |
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■ 产品详情请点击： |
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