1. TB Green Premix Ex Taq™ II(Tli RNaseH Plus)(2×Conc.) |
625 μl × 4 |
2. Mouse_Mt_primer(10 μM each) |
100 μl |
3. Mouse_genome_primer(10 μM each) |
100 μl |
4. Human_primer_Type I(CHR3)(10 μM each) |
100 μl |
5. Human_primer_Type II(CHR7)(10 μM each) |
100 μl |
6. Human_primer_Type III(CHR15)(10 μM each) |
100 μl |
7. dH2O |
1 ml×2 |
8. EASY Dilution(for Real Time PCR) |
1 ml×2 |
9. ROX Reference Dye(50×Conc.)* |
50 μl |
10. ROX Reference Dye II(50×Conc.)* |
50 μl |
11. Human Genomic DNA(100 ng/μl) |
20 μl |
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*:用于Applied Biosystems的Real Time PCR仪,用以校正孔与孔之间的荧光信号误差。
◆ 使用ROX Reference Dye 的PCR仪
·StepOnePlus Real-Time PCR System(Thermo Fisher Scientific)
◆ 使用ROX Reference Dye II的PCR仪
·Applied Biosystems 7500 Fast Real-Time PCR System(Thermo Fisher Scientific)
◆ 不使用ROX Reference Dye或ROX Reference Dye II的PCR仪
·Thermal Cycler Dice Real Time System II/Lite (Code No. TP900/TP960/TP700/TP760: 终卖)
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Limited Use Label License:[M82] |