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Media Supplements (Ndiff N2)
品牌 Code No. 产品名称 包装量 价格(元) 说明书 数量
Cellartis Y40100 NDiff® N2 5 ml ¥2,087
NDiff 细胞培养添加物
NDiff N2是成分完全确定的、不添加血清的添加物,用于组织来源的神经干细胞(tissue-derived neural stem (NS) cells)的分化获取、维持培养、定向分化。Cellartis除了传统型的NDiff N2,还提供不包含动物源成分(animal-free (AF))的NDiff N2。NDiff N2可以和与不含动物源成分的RHB-Basal一起使用,从而开发适合使用者特殊细胞类型的培养基。
NDiff N2和NDiff N2-AF含有insulin, APO-transferrin (iron poor), progesterone, putrescine, 以及sodium selenite。
 
■ 产品特点
· Completely defined, serum-free formulation
· Animal-free (AF) version contains no components of animal origin
· Use with RHB-Basal medium for optimal performance
 
产品* 规格 应用** 参考文献

NDiff N2

NDiff N2-AF

5 ml (200×) · Derivation, maintenance, and expansion of mouse ES cells
· Neural differentiation of mouse ES cells in monolayer culture
· Derivation, maintenance, and expansion of tissue-derived, adherent
   mouse and human NS cells
· Differentiation of mouse and human NS cells into functional neurons
1
*AF is the animal component-free version
**With additional supplements
 
■ 产品详情
https://www.takarabio.com/products/stem-cell-research/media-and-supplements/stem-cell-differentiation-media-and-kits/ndiff-n2
 
Mouse embryonic stem cells differentiate into neurons when cultured in adherent monoculture with NDiff N2-supplemented basal media. The differentiated cells express neuron-specific class III beta-tubulin (TuJ1, green).
 
参考文献:
1. Bottenstein JE, Cell Culture in the Neurosciences. Plenum Press (1985) New York and London.
2. Ying QL, et al. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture.
    Nature Biotechnology 21:183-186.
3. Ying QL, Smith AG. (2003) Defined conditions for neural commitment and differentiation. Methods Enzymol. 365:327-341.
4. Conti L, et al. (2005) Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biol. 3(9):e283.
5. Pollard SM, et al. (2006) Adherent neural stem (NS) cells from fetal and adult forebrain. Cereb. Cortex 16 Suppl 1:112-20.
6. Conti L, et al. (2006) Neural stem cell systems: diversities and properties after transplantation in animal models of
    diseases. Brain Pathol. (2006) 16(2):143-154.
7. Nichols J, Ying QL. (2006) Derivation and propagation of embryonic stem cells in serum- and feeder-free culture.
    Methods Mol Biol
. 329:91-98.
8. Hook L, et al. (2011) Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays.
    Neurochem Int
. 59(3): 432-44.