QuickCut Restriction Enzyme |
Specificity
5'→3‘ |
反应
温度
(℃) |
1 μl QuickCut 限制酶的酶切反应 |
热失活
条件 |
QC data |
Linear Substrate |
Plasmid DNA |
PCR Product** |
不产生Star
Activity的时间 |
Labeled
Oligo-nucleotide
Assay(LOA) |
Linear Substrate |
切断
时间
(min) |
切断
效率 |
Plasmid DNA |
切断
时间
(min) |
切断
效率 |
切断
时间
(min) |
切断
效率 |
QuickCut Afa I |
GT↓AC |
37 |
λ DNA |
5 |
100% |
pUC19 |
5 |
100% |
5 |
100% |
80℃,15 min
|
16 hr |
<10% |
QuickCut Afl II |
C↓TTAAG |
37 |
λ-Bgl II |
5 |
100% |
φX174 |
5 |
100% |
5 |
>90% |
60℃,15 min |
16 hr |
<10% |
QuickCut Alu I |
AG↓CT |
37 |
λ DNA |
5 |
100% |
pKF3 |
5 |
100% |
15 |
>80% |
70℃,15 min |
16 hr |
<10% |
QuickCut Apa I |
GGGCC↓C |
37 |
λ-BamH I |
5 |
100% |
pAdeno-X |
5 |
100% |
5 |
>80% |
80℃,15 min |
16 hr |
<10% |
QuickCut BamH I |
G↓GATCC |
30 |
λ DNA |
5 |
100% |
pUC19 |
5 |
100% |
5 |
>90% |
no* |
6 hr |
<10% |
QuickCut Bgl II |
A↓GATCT |
37 |
λ DNA |
5 |
100% |
pKF3 |
5 |
100% |
5 |
>70% |
no* |
1 hr |
<10% |
QuickCut Bln I |
C↓CTAGG |
37 |
λ-EcoR I |
5 |
100% |
pAdeno-X |
5 |
100% |
10 |
100% |
苯酚处理 |
16 hr |
<10% |
QuickCut Cla I |
AT↓CGAT |
30 |
λ DNA |
5 |
100% |
M13mp18 |
5 |
100% |
5 |
100% |
70℃,15 min |
16 hr |
<10% |
QuickCut Dpn I |
Gm6A↓TC |
37 |
pBR322-Pvu II |
5 |
90% |
pBR322 |
5 |
100% |
n.a. |
n.a. |
70℃,15 min |
16 hr |
<10% |
QuickCut Dra I |
TTT↓AAA |
37 |
λ DNA |
5 |
100% |
pBR322 |
5 |
100% |
5 |
100% |
60℃,15 min |
16 hr |
<10% |
QuickCut EcoR I |
G↓AATTC |
37 |
λ DNA |
5 |
100% |
pUC19 |
15 |
100% |
5 |
100% |
60℃,15 min |
1 hr |
<10% |
QuickCut EcoR V |
GAT↓ATC |
37 |
λ DNA |
10 |
100% |
pBR322 |
5 |
100% |
5 |
100% |
70℃,15 min |
16 hr |
<10% |
QuickCut Hae III |
GG↓CC |
37 |
λ DNA |
5 |
100% |
pUC19 |
5 |
100% |
5 |
100% |
80℃,15 min |
16 hr |
<10% |
QuickCut Hha I |
GCG↓C |
37 |
λ DNA |
5 |
100% |
pBR322 |
5 |
100% |
5 |
100% |
80℃,15 min |
16 hr |
<10% |
QuickCut Hind III |
A↓AGCTT |
37 |
λ DNA |
5 |
100% |
pUC19 |
5 |
100% |
5 |
>90% |
80℃,15 min |
16 hr |
<10% |
QuickCut Hinf I |
G↓ANTC |
37 |
λ DNA |
5 |
100% |
pUC19 |
5 |
100% |
5 |
100% |
70℃,15 min |
16 hr |
<10% |
QuickCut Hpa I |
GTT↓AAC |
37 |
λ DNA |
5 |
100% |
pKF3 |
5 |
100% |
5 |
100% |
80℃,15 min |
0.5 hr |
<10% |
QuickCut Kpn I |
GGTAC↓C |
37 |
λ-BamH I |
5 |
100% |
pUC19 |
5 |
100% |
5 |
>90% |
no* |
16 hr |
<10% |
QuickCut Mlu I |
A↓CGCGT |
37 |
λ DNA |
5 |
100% |
pKF3 |
5 |
100% |
10 |
>80% |
70℃,15 min |
16 hr |
<10% |
QuickCut Nco I |
C↓CATGG |
37 |
λ DNA |
5 |
100% |
pKF3 |
5 |
100% |
10 |
>90% |
80℃,15 min |
N/A |
<10% |
QuickCut Nde I |
CA↓TATG |
37 |
λ DNA |
5 |
100% |
pBR322 |
5 |
100% |
15 |
>90% |
70℃,15 min |
16 hr |
<10% |
QuickCut Nhe I |
G↓CTAGC |
37 |
λ-Hind III |
5 |
100% |
pBR322-Mu |
10 |
100% |
5 |
>90% |
60℃,15 min |
16 hr |
<10% |
QuickCut Not I |
GC↓GGCCGC |
37 |
pASF2-Xba I |
10 |
100% |
pASF2 |
5 |
100% |
10 |
>70% |
60℃,15 min |
N/A |
<10% |
QuickCut Pst I |
CTGCA↓G |
37 |
λ DNA |
5 |
100% |
pUC19 |
5 |
100% |
5 |
>90% |
no* |
N/A |
<10% |
QuickCut Pvu I |
CGAT↓CG |
37 |
λ-BamH I |
5 |
100% |
pBR322 |
15 |
100% |
5 |
>70% |
60℃,15 min |
16 hr |
<10% |
QuickCut Pvu ll |
CAG↓CTG |
37 |
λ DNA |
5 |
100% |
pBR322 |
5 |
100% |
30 |
>90% |
no* |
1 hr |
<10% |
QuickCut Sac I |
GAGCT↓C |
37 |
λ-EcoR I |
5 |
100% |
pUC19 |
15 |
100% |
30 |
>80% |
70℃,15 min |
16 hr |
<10% |
QuickCut Sac II |
CCGC↓GG |
37 |
pASF2-XbaI |
20 |
90% |
pASF2 |
15 |
100% |
10 |
>80% |
70℃,15 min |
16 hr |
<10% |
QuickCut Sma I |
CCC↓GGG |
30 |
λ-Hind III |
5 |
100% |
pUC19 |
5 |
100% |
5 |
>90% |
80℃,15 min |
16 hr |
<10% |
QuickCut SnaB I |
TAC↓GTA |
37 |
λ-BamH I |
5 |
100% |
M13mp18 |
5 |
100% |
30 |
>90% |
60℃,15 min |
16 hr |
<10% |
QuickCut Spe I |
A↓CTAGT |
37 |
pASF2-Xba I |
5 |
100% |
pASF2 |
5 |
100% |
30 |
>90% |
60℃,15 min |
16 hr |
<10% |
QuickCut Sph I |
GCATG↓C |
37 |
λ DNA |
5 |
100% |
pUC19 |
10 |
100% |
5 |
100% |
60℃,15 min |
N/A |
<10% |
QuickCut Stu I |
AGG↓CCT |
37 |
λ DNA |
5 |
100% |
pKF3 |
5 |
100% |
5 |
>70% |
80℃,15 min |
16 hr |
<10% |
QuickCut Sal I |
G↓TCGAC |
37 |
λ-Hind III |
20 |
100% |
pUC19 |
20 |
100% |
10 |
100% |
70℃,15 min |
4 hr |
<10% |
QuickCutSfi I |
GGCCNNNN↓
NGGCC |
50 |
pADSL2-Eco81I |
5 |
100% |
pADSL2 |
5 |
100% |
20 |
100% |
80℃,15 min |
16 hr |
<10% |
QuickCut Xba I |
T↓CTAGA |
37 |
Dam-λ-EcoR I |
5 |
100% |
pUC19 |
10 |
100% |
10 |
100% |
70℃,15 min |
16 hr |
<10% |
QuickCut Xho I |
C↓TCGAG |
37 |
λ-Hind III |
5 |
100% |
pKF3 |
10 |
100% |
10 |
>90% |
80℃,15 min |
16 hr |
<10% |
|
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