TB Green Premix Ex Taq GC*1 (2×conc.) |
625 μl × 2支 |
TUF Primer Mix (5×conc.) |
250 μl × 2支 |
dH2O |
1 ml |
ROX Reference Dye*2 (50×conc.) |
50 μl |
ROX Reference Dye II*2 (50×conc.) |
50 μl |
TUF Positive Control (1×105 Copies/μl) |
100 μl |
EASY Dilution (for Real Time PCR) |
1ml × 2支 |
|
|
*1 含有TaKaRa Ex Taq HS, dNTP Mixture, Mg2+, and TB Green。
*2 用于Applied Biosystems Real Time PCR扩增仪,用以校正孔与孔之间的荧光信号误差。
◆ 使用ROX Reference Dye的PCR仪
· StepOnePlus Real-Time PCR System(Thermo Fisher Scientific)
◆ 使用ROX Reference Dye II的PCR仪
· Applied Biosystems 7500 Fast Real-Time PCR System(Thermo Fisher Scientific)
◆ 不使用ROX Reference Dye或ROX Reference Dye II的PCR仪
· Thermal Cycler Dice Real Time System II(Code No. TP900/TP960:终卖)
· Thermal Cycler Dice Real Time System Lite(Code No. TP700/TP760:终卖) |
|