1. Fixation base solution |
13.5 ml |
Citrate buffer (pH 5.4) |
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注:首次使用前,请添加丙酮和乙醇。 |
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2. Sodium tartrate |
4 ml |
0.5 M sodium tartrate buffer (pH 5.2) |
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3. Substrate for ACP (premixed) |
for 10 ml × 3 |
NABP/FRVLB |
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4. Substrate for ALP (premixed) |
for 10 ml × 3 |
BCIP/NBT |
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5. Nuclear stain |
10 ml |
Methyl green (Contains acetic acid in the solvent.) |
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|
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试剂盒之外需要准备的试剂: |
|
· acetone (reagent grade) |
13.5 ml |
· ethanol (reagent grade) |
2.7 ml |
注:添加上述两种试剂到1. Fixation base solution中,具体操作方法请参考说明书。 |