| 人组织特异性蛋白提取物 Protein Medleys | ||||||
| 品牌 | Code No. | 产品名称 | 包装量 | 价格（元） | 说明书 | 数量 |
| Clontech | 635315 | Human Adrenal Gland Protein Medley | 100 μl | ￥2,856 |
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| Clontech | 635310 | Human Aorta Protein Medley | 100 μl | ￥2,902 |
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| Clontech | 635317 | Human Brain, amygdala Protein Medley | 100 μl | ￥2,831 |
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| Clontech | 635318 | Human Brain, frontal lobe Protein Medley | 100 μl | ￥2,831 |
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| Clontech | 635320 | Human Brain, hypothalamus Protein Medley | 100 μl | ￥2,890 |
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| Clontech | 635321 | Human Brain, Temporal Lobe Protein Medley | 100 μl | ￥2,831 |
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| Clontech | 635322 | hBrain,thalamus Protein Medley | 100 μl | ￥2,830 |
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| Clontech | 635325 | Human Brainstem Protein Medley | 100 μl | ￥2,831 |
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| Clontech | 635334 | Human Cecum Protein Medley | 100 μl | ￥2,794 |
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| Clontech | 635330 | Human Duodenum Protein Medley | 100 μl | ￥2,831 |
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| Clontech | 635329 | Human Esophagus Protein Medley | 100 μl | ￥2,890 |
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| Clontech | 635302 | Human Heart Protein Medley | 100 μl | ￥2,794 |
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| Clontech | 635332 | Human Ileum Protein Medley | 100 μl | ￥2,890 |
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| Clontech | 635331 | Human Jejunum Protein Medley | 100 μl | ￥2,830 |
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| Clontech | 635316 | Human Lymph Node Protein Medley | 100 μl | ￥2,831 |
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| Clontech | 635308 | Human Ovary Protein Medley | 100 μl | ￥2,794 |
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| Clontech | 635356 | Human Pharynx Protein Medley | 100 μl | ￥2,869 |
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| Clontech | 635355 | Human Skin Protein Medley | 100 μl | ￥2,915 |
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| Clontech | 635353 | Human Small Intestine, Mucosa Protein Medley | 100 μl | ￥2,971 |
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| Clontech | 635311 | Human Small Intestine Protein Medley | 100 μl | ￥2,794 |
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| Clontech | 635333 | Human Smooth Muscle Protein Medley | 100 μl | ￥2,831 |
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| Clontech | 635324 | Human Spinal Cord Protein Medley | 100 μl | ￥2,831 |
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| Clontech | 635313 | Human Stomach Protein Medley | 100 μl | ￥2,853 |
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| Clontech | 635314 | Human Thyroid Protein Medley | 100 μl | ￥2,890 |
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| Clontech | 635327 | Human Trachea Protein Medley | 100 μl | ￥2,831 |
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| Clontech | 635337 | Human Uterus Protein Medley | 100 μl | ￥2,831 |
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| Clontech | 635339 | Human Vagina Protein Medley | 100 μl | ￥2,794 |
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| 产品描述 | |
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Protein Medleys是指从广泛的人体组织样品中提取的组织特异性总蛋白质混合而成的pools。使用这些蛋白质提取物，研究人员可以简单直接地比较所选择的所有组织中目的蛋白质的丰度水平。Protein Medleys适用于电泳和Western Blotting检测。 Protein Medleys是在4℃条件下从全组织匀浆中制备得到的，在此过程中添加蛋白酶抑制剂，以使蛋白质水解最小化并确保尽可能多的制备组织特异性蛋白质。然后使用DNase I处理以降低粘度。所得到的Protein Medleys悬浮在由62.5 mM Tris-HCl, 2% SDS, 10% 甘油以及 0.1% 溴酚蓝 (pH6.8)组成的buffer中。每种蛋白质提取物以10 mg/ml的浓度溶解在SDS缓冲液中，每种Protein Medleys都经过聚丙烯酰胺凝胶电泳检测。 |
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| 产品特点 | |
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● 非常全面的收集了高质量的人总蛋白质提取物，可直接用于电泳检测 ● 可以快速确认目的蛋白质的组织特异性丰度 ● 容易获取难得到的人体组织，包括脑区域和胎儿组织 ● 非常适合Western Blotting检测 |
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| 应用 | |
| Western Blotting | |
| 实验例 | |
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| 使用Protein Medleys进行Western Blotting检测可以帮助我们判断目的蛋白质的大小和丰度。我们使用对应的抗体进行Western Blotting实验，通过在多种不同组织间比较，就可以确定目的蛋白质的相对丰度。本实验例使用的是anti-tubulin单克隆抗体，tublin量的变化是组织特异性合成的结果。 | |
| 泳道1：小脑 泳道2：平滑肌 泳道3：空肠 泳道4：子宫 泳道5：回肠 泳道6：脑干 泳道7：主动脉 |
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