| 应用 |
In-Fusion克隆 |
长链PCR、测序、克隆 |
克隆与表达、DNA测序 |
| 推荐产品 |
CloneAmp HiFi PCR
预混合液 |
PrimeSTAR GXL DNA
Polymerase |
PrimeSTAR Max DNA
Polymerase |
| 扩增片段大小 |
| gDNA |
﹤6 kb (human),
﹤10 kb (E. coli) |
﹤30 kb |
﹤6 kb |
| Plasmid/lambda |
﹤15 kb |
﹤40 kb |
﹤15 kb |
| cDNA |
﹤6 kb |
﹤13.5 kb |
﹤10 kb |
| 制品性能 |
5'→3' exonuclease
activity |
|
|
|
3'→5' exonuclease
activity |
√ |
√ |
√ |
T/A overhangs or
blunt |
Blunt |
Blunt |
Blunt |
| Fidelity (sequencing) |
29X Taq (GC-rich
target) |
6.5X Taq (GC-rich
target) |
29X Taq (GC-rich target) |
| Speed |
5 sec/kb |
10 sec/kb (rapid
protocol) |
5 sec/kb |
| GC-rich targets |
|
Up to 73% |
|
| 包装形式/特征 |
| Premix |
√ |
|
√ |
| Hot start |
√ |
√ |
√ |
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